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a reconstructing blog
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Tuesday, October 21, 2003

i'll always look back,
as i walk away...

Queen of my heart..izzit singing the tone in my mind? Gives me a relatively serene feeling on life..let tommorrow shine brighter than today. Oh, wat am i talking about? Ok, blog time.

Im missing alot of blogs, and i think i found the reason why. Im getting back the addiction to computers..addiction is the word. Couldnt kerb it, and it feels horrible. I literally experiences withdrawal symptoms like mood swings and a sudden high just by touching a keyboard. Got a mission now, try and resolve this bad habit. Lets kick the "old habits die hard" stuff, shall we? (hehe, and u wonder what am i doing blogging?)

Today is...bad. Ya..i guess i can describe it with bad. Yesterday was too slack, just simply do abit of cell culture. Renee gave me an idea that today will be slack too, so i came to work 10 minutes later. Obviously, she changed her mind (are all females like that?). Got back to screening today, and im gonna go home late cos i started work late. Also, my wound from yesterday's mouse dig-in-the-thumb reopened, and it was bleeding inside my glove. So sad, and it feels "suan tong" again. But funny enough, i actually got back to schedule (I kinda note the approximate timing when i do certain things) pretty soon. Ok la, not totally, but 20 minutes off track aint too bad, ya? Had a late lunch at 1:30 but it was a blessing in disguise. Saw serene today (no..it has nothing to do with "a serene feeling" as mentioned) , an old primary sch mate. She got thinner already...about 120% of pei en's thickness (haha..ironic phrase), so u can imagine how thin. Da pao, and chatted with Dr Liew abit. I find Dr Liew better to talk to, maybe because he's a guy and tends to be more slack in discussion. Renee is the details type..:( Got back and enjoyed a lonely but relaxing lunch. Got back and its work again. Im skipping the usual run gel-transfer-block-primary Ab stuff..kinda boring already. So in the middle of blocking, i asked to use the computer to check out cornell. Maybe Renee is mad at me for using the comp (hi, sensitive side)..she called me to come and i went. She went to "discuss" what am i to do for the next week etc. I regret complaining to her i got nothing to do..lol. We went into the topic of ELISA, and she said i better not do it w/o any supervision. Took that as an insult, so i replied that ELISA should be quite ok........... (note the dots). Den she got offended too and told me to get a template ready by tomolo for her to see (a challenge...) since i say i know how to do. So i diam diam, said ok and got a piece of paper to start squeezing my brain juice. Errr, i figured not enough sleep could be one reason, or i really dun know how to do ELISA (my ego refuses that fact)..but i went really awkwardly quiet, started flipping past experiment papers and blah blah blah..basically all signs of nervousness...aka: my brain was blank. I finally experienced it..total blankness. Then fear started creeping in (ok..its acutally paiseh-ness) and my mind went blank...er. Den renee got more free (she's doing something..and perhaps abit irritated to be interuppted) and the feminine kind side of women appeared. Ok, cut the story short (i can imagine myself flushing at the face), she decided to withdraw her punishment of "see la, who call u so gey kiang" and helped me planned abit. Starting, i was quite angry..found it unfair. 1stly, ELISA, we did only once or twice in yr 2, 1st semster. 2nd, the ELISA is used as a immuno technique to find dunno wth IgG1 and IgG2a, which was related to T1 and T2 helper cells. Eh, i nver boasted my immunology was perfect k? :( Also, the coating antigen concentrations used and other important factors, i could nver be able to fill in those fields. I don even have a protocol to follow! Lastly, the worst part was the planning of the wells. What to put where etc, im hopeless even at that simple idealogy. Totally humbled enough (ok..im put down by her..hai), i feel that i have to admit meekly (note....MEEKLY!) that ELISA wasnt that easy like she said. Guess she sort of accepted that apology and became more friendly. I learned two important lessons today.

1st: Poly education is limited. Even though our practical experience soars above JC students in terms of we-done-this-before...actual experiment ability is determined by how many years have you done things..aka, experience. Its like she knows alot, even though she doesnt have the mad scientist look (hehe) like Estien. Argh, she broke my confidence sia. But, i think i deserve it..:(

2nd: Boss is always right.

meng, u better not shout out with any "LOL"s .. . . . . .
=x
hahahahaha

9:14 pm