Tuesday, April 20, 2004
stress
today..went to sch to do project. We decided to pia our project. Last drug resistance to screen. DHPS. We calculated. 80 samples, 5 codons. 2 tubes for each codon (wild vs mutation). We need to do a rescreen to confirm the status of the resistance. 80*5*2*2 = ? 1600. Somebody, kill me. I think overall, our project is gonna do 3000+ PCR tubes. 1/2 of the project by 2 days..ambitous? I say an unreachable target. AHHHH
The irony? PCR is the easy part. Imagine running 1600 gel samples. lets say 1600/40. tat's like 40 gels. 1 gel 1 hr. We snatch all the gel tanks in blk 34. 1.5 hrs to load 2 gels. 1 hr to run. 2.5 hrs per 2 gels? 2 ppl do. or maybe 4 ppl do. maybe we still need 2 days. OMFG!!!!!!!!!!
WE DONT HV ENOUGH PCR MACHINES AND TANKS!! SOMEBODY SAVE MEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE!
This time, i mean it when i say "I feel like crying".
*shakes head*
it nver ends. NEVER ENDS!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
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~*BluEstAR*~ says:
dun worry lah..
~*BluEstAR*~ says:
really...
[]`xionG says:
im not worrying...im whining now
[]`xionG says:
haha
~*BluEstAR*~ says:
......
[]`xionG says:
let me whine la...wa lau
[]`xionG says:
. . . me
~*BluEstAR*~ says:
if i didn't let you whine.. i would have shut you up just now liao.... <-- note
[]`xionG says:
*breathes in
[]`xionG says:
okok, stop whining le
[]`xionG says:
anyway, where s gerard?
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ok, tat was juz now. guess wat? see the note? A halt into destressing. Whine also cannot. totally unsympathetic. was forced to scream "ahhhhh" at winnie's and py's window. Oh well, not gonna take too much offense. but heres a lesson in life. If someone screams, scream with him/her. The person will feel better (wat i wanted now)
*breaths in
i feel like swearing
2nd lesson: girls cant stand guys whining for long
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ok, i keep adding and adding. today, i really feel demoralised. one of those days. Sounds girly, but i wish there's someone for me to hug and go to bed with (not horny meaning). gonna turn in liao. tml, PCR. may they burn in hell..DNA.......
BUT!! the after-whining effect is sinkin in. Feeling prepared to tackle the hard chore tml. Work hard yixiong. Nothing brings down a man. Need be, juz screw everything else. (YES SIR!!).
*breaths in. All the best. To myself
11:31 pm